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目的:阐明河南省安阳市柯萨奇病毒A10(CV-A10)手足口病流行状况，分析其VP1基因变异和进化特征，为CV-A10病毒研究及手足口病防控工作提供依据。方法:收集2011—2015年安阳地区医院收治的手足口临床病例，获得人口流行病学信息和粪便标本，利用荧光定量PCR检测并对肠道病毒进行分型；选取CV-A10感染病例，对CV-A10 VP1基因扩增并测序、亚型鉴定、构建系统发生树、分析病毒进化特征和变异特点。结果:2011—2015年分别收集277、514、479、505和495例研究对象，检出肠道病毒阳性标本1 863份(阳性率为82.07%)。CV-A16、EV-A71、CV-A6和CV-A10为主要病毒，占肠道病毒阳性标本的94.26%，其中CV-A10阳性样本52例(占2.79%)。基于数据库CV-A10 VP1基因序列，发现CV-A10可分为A、B、C、D 4个基因型，核苷酸序列相似性为75.1%～84.2%。获得安阳地区26例样本中的CV-A10 VP1基因序列，存在2种基因亚型。结论:CV-A16和EV-A71是引起手足口病的主要病毒，CV-A6、CV-A10病原也应该引起关注和重视，近年来CV-A10的流行水平总体平稳，但存在流行水平上升的风险。“,”Objective:To clarify the epidemic situation of coxsackievirus A10 (CV-A10) hand, foot and mouth disease (HFMD) in Anyang city, Henan province and analyze the variation and evolution characteristics of VP1 gene, so as to provide the basis for the research of CV-A10 virus and the prevention and control of HFMD.Methods:From 2011 to 2015, HFMD cases were enrolled in the study. epidemiological information and fecal samples were obtained. Fluorescent quantitative PCR was used to detect and type enterovirus. The CV-A10 VP1 gene was amplified and sequenced, and the subtype was identified. Phylogenetic tree was constructed to analyze the evolution and variation characteristics of the virus.Results:A total of 2270 fecal samples (from 2011 to 2015, 277, 514, 479, 505 and 495 per year, separately). Totally 1863 enteroviruses were detected, the positive rate was 82.07%. CV-A16, EV-A71, CV-A6 and CV-A10 accounted for 94.26% of all enteroviruses. Based on all of VP1 gene sequences of CV-A10 downloaded from database, four genotypes A, B, C and D were identified. The nucleotide sequence similarity was 75.1%-84.2%. Two subtypes of CV-A10 were identified in this area.Conclusions:CV-A16 and EV-A71 are the dominant viruses causing HFMD, but attention should also be paid to the pathogens of CV-A6 and CV-A10. Although the prevalence of CV-A10 has kept stable in recent years, but there is still a risk of rising of its prevalence.