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目的探讨诱生型一氧化氮合酶(iNOS)是否参与介导BXSB小鼠狼疮肾炎(LN)及甲泼尼龙(MPS)是否可通过影响iNOS表达而缓解LN。方法用MPS对BXSB自发狼疮小鼠进行体内实验,利用反转录-聚合酶链反应(RT-PCR)和免疫组织化学技术研究了MPS对狼疮小鼠肾脏iNOS表达的影响,并观察了狼疮小鼠MPS治疗前后24h尿蛋白和NO3-/NO2-排泄量。结果BXSB狼疮小鼠肾组织检测到了iNOSmRNA的表达,而BALB/C小鼠未见表达;免疫组织化学示iNOS表达于BXSB小鼠肾小管上皮细胞、血管内皮细胞、肾间质及肾小球浸润的炎症细胞胞质内。MPS治疗组BXSB狼疮小鼠肾组织iNOSmRNA表达量显著低于未治疗组(P<0.01);治疗组24h尿蛋白及NO3-/NO2-排泄量显著低于未治疗组(P<0.01,P<0.05)。结论iNOS及其合成的一氧化氮(NO)可能参与介导了LN。MPS可能通过抑制肾组织iNOS的表达缓解LN。
Objective To investigate whether inducible nitric oxide synthase (iNOS) participates in the mediation of LN induced by iNOS in mice with lupus nephritis (LN) and methylprednisolone (MPS) in BXSB mice. Methods The spontaneous lupus mice with BXSB were treated with MPS in vivo. The effect of MPS on the expression of iNOS in kidney of lupus mice was studied by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. 24h urinary protein and NO3- / NO2- excretion before and after MPS treatment. Results The expression of iNOS mRNA was detected in the kidney of BXSB lupus mice but not in BALB / C mice. Immunohistochemistry showed that the expression of iNOS in tubular epithelial cells, vascular endothelial cells, renal interstitium and glomerular infiltration in BXSB mice Of inflammatory cells within the cytoplasm. The expression of iNOSmRNA in kidney of BXSB lupus mice in MPS treatment group was significantly lower than that in untreated group (P <0.01), while the urinary protein and NO3- / NO2- excretion in treatment group was significantly lower than that of untreated group (P <0.01, P < 0.05). Conclusion iNOS and its synthesis of nitric oxide (NO) may be involved in the mediation of LN. MPS may relieve LN by inhibiting the expression of iNOS in renal tissue.