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草铵膦(glufosinate-ammonium,GLA)是一种广谱触杀型非选择性除草剂,随着在生产中应用的不断增加,其生物安全性也越来越受到人们的重视。试验以斑马鱼Danio rerio胚胎为动物模型,用不同剂量的草铵膦染毒受精后2 h的斑马鱼胚胎,观察其死亡率和形态学变化,并应用原位杂交和QRT-PCR技术检测了Vasa基因表达情况,以分析草铵膦的毒性作用。结果表明:1.6μg/L的草铵膦对斑马鱼胚胎有显著致死作用、1.3μg/L的草铵膦有显著致畸作用,其中畸形表现为尾部弯曲和阻碍黑色素沉着。原位杂交结果显示:1.3μg/L的草铵膦可引起Vasa表达的部分缺失,QRT-PCR定量分析发现,草铵膦可引起斑马鱼胚胎Vasa基因表达水平下调3.8倍。表明草铵膦对斑马鱼胚胎有较强的致死毒性、致畸作用和潜在的生殖毒性。
Glufosinate-ammonium (GLA) is a kind of broad-spectrum contact non-selective herbicide. With the increasing use of GLA, its biosafety has drawn more and more attention. In this study, zebrafish Danio rerio embryo was used as an animal model. Zebrafish embryos were infected with glufosinate at different doses for 2 h. The mortality and morphological changes were observed. In situ hybridization and QRT-PCR Vasa gene expression to assess the toxic effects of glufosinate. The results showed that: 1.6μg / L of glufosinate have a significant lethal effect on zebrafish embryos, 1.3μg / L glufosinate has a significant teratogenic effect, which deformity showed tail curvature and hinder melanin deposition. In situ hybridization results showed that glufosinate of 1.3μg / L could cause partial deletion of Vasa expression. Quantitative analysis with QRT-PCR showed that glufosinate-induced phosphorylation of Vasa gene in zebrafish embryo was down-regulated by 3.8-fold. The results showed that glufosinate had strong virulence, teratogenicity and potential toxicity to zebrafish embryos.