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为探讨 8 羟基 脱氧鸟苷 (8 OH dG)与人群肺癌发生及癌变相关基因的关系 ,使用针对 8 OH dG加合物的鼠抗人单克隆抗体 ,通过免疫学方法检测 1 50例肺癌、1 2 0例癌旁肺组织、40例肺良性病变和 40例正常肺组织中氧化损伤标志物 8 OH dG的含量 ;同时检测相应肺组织中P53、C MYC、K RAS、BCL 2、hTERT等癌变相关基因的表达 ,分析 8 OH dG与上述癌变相关基因表达的关系。结果显示 ,92 7% (1 39 1 50 )的肺癌组织中可检出氧化损伤标志物 8 OH dG ,其阳性标志细胞的比率为 (2 4 0 0± 2 5 1 1 ) % ;1 7 5 % (2 1 1 2 0 )的癌旁肺组织可检出 8 OH dG ,阳性标志细胞的比率为 (2 42± 5 98) % ;1 0 0 % (4 40 )的肺良性病变组织可检出 8 OH dG ,阳性标志细胞的比率为 (0 80± 1 30 ) % ;5 0 % (2 40 )的正常肺组织可检出 8 OH dG加合物 ,阳性标志细胞的比率为 (0 34± 1 0 1 ) % ,肺癌中 8 OH dG的含量高于其它肺组织 (P <0 0 1 )。将肺癌病例按性别、年龄、细胞类型和吸烟史分层 ,它们皆与 8 OH dG无关联。分析 8 OH dG与癌变相关基因的关系 ,发现肺癌组织K RAS、BCL 2基因阳性表达者 8 OH dG的含量高于上述基因阴性表达者 ,P值分别为 0 0 35和 0 0 34 ;而P53、C MYC和hTERT基因表达与 8 OH dG的含
In order to investigate the relationship between 8-OH-deoxyguanosine (8-OH-dG) and lung cancer occurrence and carcinogenesis, a mouse anti-human monoclonal antibody against 8-OH dG adduct was used to detect 150 lung cancer patients by immunological method 20 cases of paracancer lung tissue, 40 cases of benign lung disease and 40 cases of normal lung tissue oxidative damage markers 8 OH dG content; simultaneous detection of the corresponding lung tissue P53, C MYC, K RAS, BCL 2, hTERT other carcinogenesis Related gene expression, analysis of 8 OH dG and cancer-related gene expression. The results showed that 8 OH dG, a marker of oxidative damage, was found in 92 7% (1 391 50) lung cancer tissues with a positive rate of (2400 ± 2 5 1 1)% There was 8 OH dG in the para-carcinoma tissue adjacent to the tumor (%), and the percentage of positive cells was (42 ± 5 98)%. In 100% (4 40) of benign lung tissues, The percentage of positive labeled cells was (80 ± 1 30)% at 8 OH dG, while that of 8 OH dG adduct was detectable at 5 0% (2 40) of normal lungs ± 1 0 1)%, 8 OH dG in lung cancer was higher than that in other lung tissues (P <0.01). Lung cancer cases were stratified by sex, age, cell type and smoking history, and none of them was associated with 8 OH dG. The relationship between 8-OH-dG and cancer-related genes was analyzed. The results showed that the content of 8-OH-dG in K RAS and BCL2 gene in lung cancer was higher than that in the above-mentioned genes, P values were 0 0 35 and 0 0 34, respectively. , C MYC and hTERT gene expression and 8 OH dG containing