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目的:从分子生物学基础上了解同一次流行、不同来源的霍乱弧菌之间的遗传相关性,为有效控制传染源提供依据。方法:采用PCR技术对2005年分离的70株霍乱弧菌检测CT基因后,进行PFGE分型研究。结果:分离自患者O1、O139群霍乱弧菌均有CT基因,而分离自外环境1株O1群稻叶菌株及1株O139菌株CT基因为阴性。所试菌株PFGE分型共呈3大亚类8种带型。结论:霍乱流行期间分离于患者的霍乱弧菌PFGE图谱基本一致,与外环境分离的非流行株在分化关系上较远。
OBJECTIVE: To understand the genetic correlation between the same epidemic and different origins of Vibrio cholerae based on molecular biology, and to provide evidence for effective control of infection sources. Methods: The CT genotypes of 70 V. cholerae isolated in 2005 were detected by PCR. Results: The CT genes were isolated from Vibrio cholerae O1 and O139 isolates from patients. The isolates of O1 isolates from O1 group were negative for CT genes. The strains of PFGE were tested in 8 major subtypes of 8 belt types. CONCLUSIONS: The Vibrio cholerae PFGE patterns isolated from patients during the cholera epidemic are basically identical, with non-epidemic strains isolated farther away from the external environment.