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用小鼠血糖法测定胰岛素生物效价,属于量反应,误差较小,节省动物。葡萄糖氧化酶和过氧化酶试剂法实验简单,观察指标是比色得到的吸收度,实际小鼠血糖值由葡萄糖标准曲线回归方程换算得出,以mg/100ml血为单位,葡萄糖含量为反应值计算。实验设计采用拉丁方设计,该设计的可靠性测验方差统计分析和效价计算及误差估计见另文。由于拉丁方设计的统计分析,数据运算步骤较多,复杂繁琐,有必要借助电子计算机这一工具,以便能迅速而又准确地计算出实验结果,对于推广使用这一方法,提高工作效率,将有一定的
Insulin biological titer was determined by mouse blood glucose method, which belongs to the quantitative reaction with small error and saves the animal. Glucose oxidase and peroxidase reagent method is simple, the observation index is colorimetric absorbance, the actual mouse blood glucose value is derived from the glucose standard curve regression equation, in mg / 100ml blood as a unit, the glucose content of the reaction value Calculate. Experimental design using Latin square design, the reliability of the design statistical analysis of variance and valence calculations and error estimates see another article. Due to the statistical analysis of the Latin square design, data computing steps more complex and cumbersome, it is necessary to use the electronic computer as a tool to quickly and accurately calculate the experimental results for the promotion of the use of this method to improve work efficiency will be There is some