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[目的]建立一种高效液相色谱法测定氟硅唑·丙环唑混合药剂有效成分含量的定量分析方法。[方法]采用高效液相色谱法,以甲醇和水(体积比85∶15)为流动相,流速为1 m L/min,以220 nm为检测波长,对混合样中氟硅唑和丙环唑进行液相色谱分离测定。[结果]氟硅唑和丙环唑保留时间分别为4.627、5.640 min,线性相关系数分别为0.9999和0.9983,标准偏差分别为0.0873、0.0707,变异系数分别为0.3363%、0.0945%;平均回收率分别为98.80%、97.34%。[结论]该方法操作简便,分离效果好,精密度与准确度高,可同时测定氟硅唑和丙环唑含量。
[Objective] To establish a method for the quantitative analysis of the active ingredient in flusilazole and propiconazole mixture by HPLC. [Method] The mobile phase consisted of methanol and water (volume ratio 85:15) with the flow rate of 1 m L / min and the detection wavelength of 220 nm as the mobile phase by high performance liquid chromatography (HPLC) Azole was subjected to liquid chromatography. [Results] The retention times of flusilazole and propiconazole were 4.627 and 5.640 min, respectively. The linear correlation coefficients were 0.9999 and 0.9983, respectively. The standard deviations were 0.0873 and 0.0707, respectively. The coefficients of variation were 0.3363% and 0.0945% 98.80%, 97.34%. [Conclusion] The method is simple and easy to operate with good separation effect and high precision and accuracy. The content of flusilazole and propiconazole can be determined simultaneously.