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为探讨山东省亚急型克山病患儿与柯萨奇B组病毒的关系,用缺口平移法制备生物素标记的COXB_3病毒的cDNA探针,与亚急型克山病病例尸检心肌组织9例及15例脑外伤,CO中毒等意外死亡的正常成人心肌组织和10例非病区7～8个月胎儿心组织切片进行原位杂交,结果9例山东亚急型克山病有5例检测到COXB组病毒的RNA,阳性率为55.5％(5/9),而对照组均为阴性。结果提示COXB组病毒感染是山东省亚急型克山病发病原因之一。
To investigate the relationship between children with subacute KK in Shandong province and Coxsackie B virus, a cDNA probe encoding biotin-labeled COXB_3 virus was prepared by nick translation. Cases and 15 cases of traumatic brain injury, CO poisoning and other accidental death of normal adult myocardial tissue and 10 cases of non-ward 7-8 months of fetal heart tissue sections in situ hybridization results 9 cases of subacute Keshan disease in Shandong Province in 5 cases The positive rate of COXB virus RNA was 55.5% (5/9), while the control group was negative. The results suggest that COXB group virus infection is one of the causes of subacute Keshan disease in Shandong Province.