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以静电纺制备的PAN纳米纤维为原料,用盐酸羟胺水溶液对其进行化学改性后得到偕胺肟基PAN螯合纳米纤维(AOPAN),将其与Cu~(2+)配位得到金属配合纳米纤维为载体固定漆酶,并研究了固定漆酶对对苯二酚的降解。分别采用红外光谱仪(FT-IR)、扫描电子显微镜(SEM)和能谱仪(EDAX)对不同处理阶段的PAN纳米纤维进行了观察分析,并对固定漆酶的性能进行了研究,通过测定不同降解阶段对苯二酚的吸光度变化来分析其降解情况。结果表明:改性后的PAN纳米纤维表面没有出现严重的裂痕或降解现象。固定漆酶的最适pH值为4,最适温度为50℃。铜离子配合螯合PAN纳米纤维膜(Cu-AOPAN)固定的漆酶,3次重复利用后,对对苯二酚的降解率分别为55.18%、50.75%、50.65%。
PAN nanofibers prepared by electrospinning were used as raw materials and chemically modified with hydroxylamine hydrochloride to obtain amidoxime-PAN chelated nanofibers (AOPAN), which coordinated with Cu 2+ to obtain a metal complex Nanofibers immobilize laccase and immobilized laccase on hydroquinone degradation. PAN nanofibers in different treatment stages were observed and analyzed by FT-IR, SEM and EDAX, respectively. The properties of immobilized laccase were studied. The degradation of hydroquinone absorbance changes to analyze its degradation. The results show that there are no serious cracks or degradation on the surface of modified PAN nanofibers. The optimum laccase pH value of 4, the optimum temperature of 50 ℃. The degradation rates of hydroquinone were 55.18%, 50.75% and 50.65% respectively after copper ions and chelated PAN nanofibrous membrane (Cu-AOPAN) immobilized laccase were used three times.