I was fortunate to work with/for Dr Britton Chance as his postdoctoral fellow, in the Biochemistry and Biophysics Department at the University of Pennsylvania, between August 1991 and January 1994. As anyone who worked for him, I had a sufficient dosage o
In the past two decades, two-photon microscopy (TPM) transforms biomedical research, allowing nondestructive high-resolution fluorescent molecular imaging and label-free imaging in vivo and in real time. Here we review the recent advances of TPM technolog