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研究果实含酸量的遗传特性及其酸度基因的分子标记可以为果品品质育种提供辅助手段。以果实低酸的’短枝富士’(Spur Fuji)和高酸的’粉红女士’(Pink Lady)F1代群体(216株)为试材,利用SSR(simple sequence repeat)技术,结合集群分类分析法(bulked segregation analysis,BSA)进行了苹果酸度基因(Ma)分子标记研究。经过102对SSR引物的筛选,获得了与果实酸性状紧密连锁的分子标记CH03d12104和CH03d12118两个位点,连锁距离分别为3.24和2.31cM。分析表明Ma1与Ma2对果实含酸量有控制作用,Ma对ma表现为完全显性。
Studying the genetic characteristics of fruit acid content and the molecular markers of its acidity genes can provide supplementary means for the breeding of fruit quality. Spur Fuji (low-acidity) and F1 generation (high-acidity Pink Lady) population (216 strains) were used as materials, SSR (simple sequence repeat) technology and cluster analysis (MA) molecular marker of bulked segregation analysis (BSA). After screening of 102 pairs of SSR primers, molecular markers CH03d12104 and CH03d12118 closely linked to fruit acid traits were obtained, with linkage distances of 3.24 and 2.31cM, respectively. Analysis showed that Ma1 and Ma2 had fruit acid content control, Ma ma completely showed dominant.