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目的应用不同频率的电刺激海人酸模型癫痫大鼠STN核,观察STN核以及SNr(黑质网状部)神经元细胞在刺激后放电频率的变化,研究电刺激STN对STN内神经元和SNr神经元放电的影响,探讨STN-DBS治疗癫痫的作用机制。方法10只癫痫大鼠为实验组,另10只正常大鼠作为对照组。参照大鼠立体定向图谱,将记录的玻璃微电极和刺激电极分别插入STN、SNr核团内,刺激频率分为三组,分别为30 Hz、130 Hz、260 Hz。通过单神经元放电细胞外记录方法分别于高频刺激前后记录脑内核团神经元放电情况,分析神经元在STN-HFS刺激前和刺激时放电改变情况。结果正常大鼠的STN及SNr神经元放电与癫痫模型大鼠相比,两者放电频率不存在显著性差异,对放电模式的分析发现两者也无明显差异(P>0.05)。癫痫大鼠的STN及SNr神经元在30 Hz的刺激过程中放电频率多数没有明显变化。随着放电频率的增加两种神经元在电刺激后多数神经元放电明显受到抑制。在130 Hz和260 Hz组,受抑制的神经元较30 Hz组明显增加,具有显著性差异(P<0.05)。结论本研究证实高频电刺激STN明显抑制了STN和SNr神经元的兴奋性,其效果与频率是相关的。推测STN-HFS的作用机制可能通过对STN神经元兴奋性的抑制,调节相关联系核团的输出。
Objective To investigate the effect of STN stimulation on STN nucleus in STN nucleus and SNr (substantia nigra reticulum) neurons after electrical stimulation of STN in hippocampal neurons of KA model rats. SNr neurons discharge to explore the mechanism of STN-DBS treatment of epilepsy. Methods Ten epileptic rats were used as experimental group and the other 10 normal rats as control group. Reference to the rat stereotactic map, the recorded glass microelectrode and stimulation electrode were inserted into the STN, SNr nucleus, the stimulation frequency is divided into three groups, respectively, 30 Hz, 130 Hz, 260 Hz. Single neuron discharge extracellular recording method were recorded before and after high-frequency stimulation of brain nuclear discharge neurons, neurons in the STN-HFS stimulation before and during stimulation changes. Results Compared with epilepsy model rats, the discharge frequency of STN and SNr neurons in normal rats did not show significant difference. There was no significant difference between the two groups in the discharge patterns (P> 0.05). In STN and SNr neurons of epileptic rats, the discharge frequency did not change significantly at 30 Hz. With the increase of discharge frequency, the discharge of most neurons in both neurons was significantly inhibited after electrical stimulation. In the 130 Hz and 260 Hz groups, the inhibited neurons increased significantly compared with the 30 Hz group, with significant difference (P <0.05). Conclusions This study demonstrates that high frequency electrical stimulation of STN significantly inhibits the excitability of STN and SNr neurons, and its effect is correlated with frequency. It is speculated that the mechanism of action of STN-HFS may regulate the output of related related nuclei by inhibiting the excitability of STN neurons.