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用放免分析（ＲＩＡ）法监测了急性脑缺血及再灌注条件下动物血及脑匀浆催乳素（ＰＲＬ）、生长激素（ＧＨ）及β－内啡肽（β－ＥＰ）的活性变化。发现：急性全脑缺血及缺血３０ｍｉｎ再灌注组血及脑内ＰＲＬ、ＧＨ与β－ＥＰ活性显著高于对照组和不完全脑缺血组（Ｐ均＜０．０５）。提示：血与脑内ＰＲＬ、ＧＨ及β－ＥＰ的活性变化与急性全脑缺血－再灌注的发生、发展有其内在联系，反映脑缺血、缺氧条件下中枢神经系损伤的严重程度。此外，脑匀浆ＰＲＬ、ＧＨ及β－ＥＰ含量以下丘脑最丰富，提示：下丘脑与急性全脑缺血及再灌注条件下机体神经内分泌机制紊乱密切相关。急性脑血循环障碍条件下，积极对ＰＲＬ、ＧＨ及β－ＥＰ的动态监测，对于及时到正神经内分泌机制紊乱、稳定机体内环境、减少脑血管病的并发症和提高脑血管病的整体防治水平具有实际指导意义。
Radioimmunoassay (RIA) was used to detect the changes of the activities of prolactin (PRL), growth hormone (GH) and β-endorphin (β-EP) in blood and brain homogenate of rats under acute cerebral ischemia and reperfusion. The results showed that the activities of PRL, GH and β-EP in blood and brain after acute global cerebral ischemia and ischemia-reperfusion were significantly higher than those in control group and incomplete cerebral ischemia group (all P <0.05). It is suggested that the changes of the activity of PRL, GH and β-EP in blood and brain are intrinsically related to the occurrence and development of acute global cerebral ischemia-reperfusion and reflect the severity of central nervous system injury under cerebral ischemia and hypoxia . In addition, brain homogenate PRL, GH and β-EP content in the hypothalamus is the most abundant, suggesting that: the hypothalamus and acute global cerebral ischemia and reperfusion conditions, the neuroendocrine mechanism is closely related to disorder. Under the condition of acute cerebral blood circulation disorder, the dynamic monitoring of PRL, GH and β-EP is of great significance for the timely prevention of neuroendocrine disorders, stabilization of the internal environment, reduction of complication of cerebrovascular disease and improvement of the overall prevention and treatment of cerebrovascular disease Practical significance.