Optimization of Two-Dimensional Gel Electrophoresis for Kenaf Leaf Proteins

• 作 者: CHEN Tao QI Jian-min XU Jian-t
• 机 构: KeyLaboratoryforGenetics
• 出 处: 中国农业科学：英文版
• 发表日期: 2011年12期
• 关键词: 二维凝胶电泳 叶蛋白 红麻 聚丙烯酰胺凝胶电泳 优化 固相PH梯度 提取方法 双向凝胶电泳 kenaf protein extraction proteom
• 基金项目: Acknowledgements This work was supported by the Project of Fiber Crops Industrial System Construction in China （nycytx-19- E05）, the Natural Public Welfare Sector Projects of China （nyhyzx07-018）, and

To establish a suitable and effective protocol of protein extraction for two-dimensional gel electrophoresis (2-DE) analysis in kenaf leaf tissues, three extraction methods (trichloroacetic acid/acetone, urea/thiourea, and phenol extraction methods) were