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目的:观察健脑通胶囊对AD大鼠一氧化氮合酶(NOS)含量的影响。方法:84只SD大鼠,随机挑出12只,作为空白组,剩余72只给予AD模型复制,造模成功后死亡3只,剩余69只随机分成5组:AD模型组(12只)、脑复康组(12只)、健脑通胶囊小剂量组(15只)、健脑通胶囊中剂量组(15只)、健脑通胶囊大剂量组(15只)。健脑通胶囊大、中、小剂量组分别以剂量0.94g·kg-1·d-1、0.47g·kg-1·d-1、0.235g·kg-1·d-1灌胃;脑复康组予以50mg/mL的脑复康片混悬液按0.162g·kg-1·d-1的剂量灌胃,空白组、模型组予以等量生理盐水灌胃,每天上午给药1次,连续给药30d。测定各组大鼠脑组织NOS含量。结果:健脑通胶囊各组与脑复康组NOS含量均高于模型组(P<0.05);且健脑通胶囊大、中、小剂量组间比较,差异均有统计学意义(P<0.05,P<0.01)。结论:健脑通胶囊能减少AD模型大鼠NOS含量,延缓老年痴呆的发展,有效保护脑功能,且有一定的量效关系。
Objective: To observe the effect of Jiannaotong Capsule on the content of nitric oxide synthase (NOS) in AD rats. Methods: Eighty - four SD rats were randomly selected. Twelve mice were randomly selected as the blank group. The remaining 72 rabbits were given AD model. Three rabbits died after successful modeling. The remaining 69 rabbits were randomly divided into 5 groups: AD model group (12 rats) Naofukang group (n = 12), Jiannaotong capsule low dose group (n = 15), Jiannaotong capsule middle dose group (n = 15) and Jiannaotong capsule high dose group (n = 15). Jiannaotong Capsules were given intragastric administration of 0.94g · kg-1 · d-1, 0.47g · kg-1 · d-1, and 2.35g · kg-1 · d-1, respectively, Rehabilitation group was given 50mg / mL Naofushang tablets suspension by 0.162g · kg-1 · d-1 dose of gavage, the blank group, the model group was given the same amount of saline gavage once a day in the morning , Continuous administration 30d. The content of NOS in brain tissue of each group was determined. Results: The NOS contents of Jiannaotong Capsules and Naofukang group were higher than those of model group (P <0.05), and the difference was statistically significant between Jiannaotong Capsules groups (P < 0.05, P <0.01). Conclusion: Jiannaotong Capsule can reduce the NOS content of AD model rats, delaying the development of Alzheimer ’s disease and effectively protecting brain function, and has a certain dose - effect relationship.