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目的:研究和观察天然孕酮阴道环的长效避孕效果,及其在山羊体内的血药浓度和持续释放时间。方法:①将24只具有生育能力的新西兰雌兔随机分为4组,分别阴道置入空白环、天然孕酮阴道环低剂量(210 mg/环)、中剂量(420 mg/环)和高剂量(840 mg/环)。手术15 d后进行交配,观察天然孕酮阴道环对家兔的长效避孕效果。②将12只去卵巢山羊随机分为2组,分别给予国产和智利产天然孕酮阴道环,并于置入阴道环前、后不同时间点颈静脉采血,用磁性分离酶联免疫法测定血清中天然孕酮的浓度。结果:①阴道环对动物的一般状况均无明显影响,高剂量组和中剂量组避孕效果较好,能维持3个月,而低剂量组避孕效果较差。②国产和智利产阴道环血药浓度除20 d、27 d、90 d外无显著性差异(P>0.05)。结论:天然孕酮阴道环高、中剂量组均有长效避孕作用;与智利孕酮阴道环相比,国产天然孕酮阴道环放置在山羊阴道后的血药浓度变化无明显差异,其维持释放剂量和时间相当。
Objective: To study and observe the long-term contraceptive effect of natural progesterone vaginal ring and its plasma concentration and sustained release time in goats. Methods: ①Twenty-four New Zealand female rabbits with reproductive capacity were randomly divided into 4 groups: vaginal instillation with blank ring, low dose of natural progesterone vaginal ring (210 mg / ring), medium dose (420 mg / Dose (840 mg / ring). After 15 days of operation, mating was performed to observe the long-term contraceptive effect of natural progesterone vaginal ring on rabbits. Twelve ovariectomized goats were randomly divided into 2 groups, and were given natural progesterone-producing vaginal ring of domestic and Chile respectively. Blood samples were collected from the jugular vein at different time points before and after insertion into the vaginal ring. The serum was separated by enzyme-linked immunosorbent assay The natural progesterone concentration. Results: ①Vaginal ring had no significant effect on the general condition of animals, contraception in high dose group and middle dose group was better, which could maintain for 3 months, while contraception in low dose group was less effective. ② Domestic and Chile vaginal ring blood concentration in addition to 20 d, 27 d, 90 d no significant difference (P> 0.05). Conclusion: Compared with Chilean progesterone vaginal ring, the natural progesterone vaginal ring vaginal ring placed in the goat vaginal blood concentration after the change was no significant difference, the maintenance of natural progesterone vaginal ring high and medium dose groups have long-acting contraceptive effect Release dose and time quite.