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采用菌丝生长速率法测定了不同地区115个辣椒疫霉菌株对双炔酰菌胺的敏感性,同时测定了紫外诱导和药剂驯化的10个抗性突变体及其4个亲本菌株对双炔酰菌胺、烯酰吗啉、甲霜灵、嘧菌酯和霜脲氰的敏感性。山东青州、寿光和河北定兴、藁城4地辣椒疫霉菌株均对双炔酰菌胺较敏感,且敏感性差异不显著。不同抗性水平菌株对5种药剂的敏感性测定和相关性分析结果显示,双炔酰菌胺与其它几类药剂之间均不存在交互抗性。10个抗性突变体继代培养11代后,8个抗性突变体抗药性不能稳定遗传;2个抗性突变体抗药性不仅能够稳定遗传,且EC50值维持在很高水平,分别为1 429.524μg/mL和6 872.476μg/mL,因此,双炔酰菌胺的连续使用有利于抗药群体的发展。
The sensitivity of 115 capsici strains to mandipropamid in different areas was determined by mycelial growth rate method. Ten resistant mutants induced by ultraviolet induction and domestication of the medicament were also determined. Amikacin, dimethomorph, metalaxyl, azoxystrobin and cymoxanil. Shandong Qingzhou, Shouguang and Hebei Dingxing, Yuncheng 4 Phytophthora capsici strains are more sensitive to mandipropamid, and the difference was not significant. The results showed that there was no cross-resistance between mandipropamid and several other agents. Ten resistant mutants were subcultured for eleven generations. The resistance of the eight resistant mutants was not stable and stable. The resistance of the two resistant mutants was stable and the EC50 values were very high 429.524 μg / mL and 6 872.476 μg / mL, therefore, the continuous use of mandipropamid favored the development of drug-resistant populations.