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目的探讨生理性深层海水(PDSW)对45.0℃高温环境动物耐受性的影响及机制。方法将取自我国海南省海域的深层海水进行制备,制成PDSW,检测所含有的部分元素。20只昆明小鼠,雌雄各半,分为对照组和实验组,对照组小鼠饮用自来水,实验组饮用PDSW,连续15d,然后把各组小鼠放入45.0℃高温环境下饲养,记录每只小鼠死亡时间,直至各组小鼠全部死亡为止。并取每只小鼠脑、肺、心、肝和肾做病理检查。结果无论是雌性小鼠还是雄性小鼠,实验组的小鼠在45.0℃高温下的生存时间比对照组的小鼠长,差异有统计学意义(P<0.05)。并且雌性小鼠的生存时间要比雄性小鼠的生存时间长,差异有统计学意义(P<0.05)。组织学分析表明PDSW对实验小鼠的脑、心、肺、肝、肾等器官均有保护作用。Western blot检测发现,实验组小鼠肝组织HSP72表达较对照组强,差异有统计学意义(P<0.05)。结论 PDSW可以增强小鼠对45.0℃高温环境的耐受性,其机制考虑与PDSW促进热休克蛋白表达有关。
Objective To investigate the effect and mechanism of physiological deep seawater (PDSW) on animal tolerance at high temperature of 45.0 ℃. Methods Deep seawater from the sea area of Hainan Province of China was prepared and made into PDSW to test some elements contained. 20 Kunming mice, male and female, divided into control group and experimental group, the control group of mice drinking tap water, the experimental group drinking PDSW, continuous 15d, and then the mice into 45.0 ℃ high temperature environment, keeping record Only mouse death time, until all mice died so far. And take each mouse brain, lung, heart, liver and kidney pathological examination. Results The survival time of the mice in the experimental group was longer than that in the control group (P <0.05), no matter in the female mice or in the male mice. And the survival time of female mice than male mice longer survival time, the difference was statistically significant (P <0.05). Histological analysis showed that PDSW had protective effects on the brain, heart, lung, liver, kidney and other organs in experimental mice. The results of Western blot showed that the expression of HSP72 in experimental group was higher than that in control group (P <0.05). Conclusion PDSW can enhance the tolerance of mice to high temperature of 45.0 ℃. The mechanism of PDSW is related to the promotion of heat shock protein expression by PDSW.