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目的 观察活化的小胶质细胞 (表达主要组织相容性抗原 )在实验性自身免疫性脑脊髓炎 ( EAE)大鼠脊髓中的变化 ,探讨 EAE大鼠发病相关生物学机制。方法 采用免疫组化法观察豚鼠全脊髓匀浆诱导的 Wistar大鼠 EAE过程中脊髓内表达 MHC 、MHC 类抗原的小胶质细胞的变化情况。结果 对照组脊髓内未发现表达 MHC抗原细胞 ,实验组脊髓内表达 MHC 抗原细胞与表达 MHC 抗原细胞的分布和形态一致 ,小胶质细胞变化与 EAE大鼠的病程一致。动物临床症状评分 2分和 3分 EAE大鼠脊髓表达 MHC抗原的小胶质细胞比评分 1分大鼠明显增高 ( P <0 .0 1 ) ,恢复期 EAE大鼠表达 MHC抗原的小胶质细胞明显减少 ( P <0 .0 1 )。结论 活化小胶质细胞与 EAE大鼠的病情相关 ,提示其可能通过表达 MHC抗原在 EAE大鼠的发病机制中具有作用
Objective To observe the changes of activated microglia (express major histocompatibility antigen) in the spinal cord of experimental autoimmune encephalomyelitis (EAE) rats and to explore the biological mechanism of EAE. Methods The changes of microglial cells expressing MHC and MHC antigens in the spinal cord during the EAE of Wistar rats induced by whole spinal cord homogenate were observed by immunohistochemistry. Results No MHC antigen was expressed in the spinal cord of the control group. The distribution and morphology of MHC antigen-expressing cells in the spinal cord of the experimental group were the same as those of the MHC antigen-expressing cells. The microglial changes were consistent with those of the EAE rats. Animal clinical symptom score The microglial cells expressing MHC antigen in the spinal cord of 2 and 3 EAE rats were significantly higher than the score of 1 (P <0.01), while the microglia expressing MHC antigen in recovery EAE rats Cells were significantly reduced (P <0.01). Conclusion Activated microglial cells are associated with the pathogenesis of EAE rats, suggesting that they may play a role in the pathogenesis of EAE rats by expressing MHC antigen