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目的:探讨叔丁基对苯二酚(t BHQ)和莱菔硫烷(SFN)在患有创伤性脑损伤大鼠的疗效差异性。方法:80只健康成年的雄性SD大鼠分为假手术组、常规损伤组、t BHQ治疗组和SFN治疗组,使用电子颅脑损伤仪(e CCI)制备TBI模型。其中t BHQ治疗组在伤前24 h大鼠腹腔注射三次t BHQ(50 mg/kg),每8 h一次;SFN治疗组在伤后15 min给予腹腔注射SFN(5 mg/kg)。给药24 h后,采用m NSS方法评价各组大鼠神经功能缺损状况,利用干湿称量法计算脑含水量,Western blot和ELISA方法分别测定大鼠脑组织的NOX2和Nrf2的表达水平。结果:损伤发生后第24 h,t BHQ治疗组和SFN治疗组在m NSS评分((4.5±0.71)vs(9.2±0.79),(6.0±0.82)vs(9.2±0.79))、脑水肿(79.4%vs 85.6%,80.3%vs 85.6%)、NOX2和Nrf2(0.93 ng/m L vs 0.81 ng/m L,0.87 ng/m L vs 0.81 ng/m L)表达上与常规损伤组差异明显,而t BHQ治疗组和SFN治疗组间在m NSS评分((4.5±0.71)vs(6.0±0.82))、NOX2和Nrf2(0.93 ng/m L vs 0.87 ng/m L)表达上差异显著。结论:在大鼠TBI模型中,t BHQ和SFN均可以有效的降低机体自身的氧化应激作用,并改善神经功能,但t BHQ的疗效要好于SFN。
OBJECTIVE: To investigate the differences in the therapeutic effects of t-butyl hydroquinone (t BHQ) and sulforaphane (SFN) in rats with traumatic brain injury. Methods: Eighty healthy adult male Sprague-Dawley rats were randomly divided into sham operation group, conventional injury group, t BHQ treatment group and SFN treatment group. The TBI model was prepared by electronic brain injury instrument (e CCI). The rats in t BHQ group were injected intraperitoneally with t BHQ (50 mg / kg) 24 h after injury, once every 8 hours. The SFN group received intraperitoneal injection of SFN (5 mg / kg) 15 minutes after injury. After 24 h, m NSS was used to evaluate the neurological deficit in each group. The brain water content was calculated by wet and dry method. The expression levels of NOX2 and Nrf2 in rat brain were measured by Western blot and ELISA respectively. RESULTS: At 24 h after injury, there was significant difference in m NSS score ((4.5 ± 0.71) vs (9.2 ± 0.79), (6.0 ± 0.82) vs (9.2 ± 0.79), cerebral edema 79.4% vs 85.6%, 80.3% vs 85.6%, NOX2 and Nrf2 (0.93 ng / m L vs 0.81 ng / m L, 0.87 ng / m L vs 0.81 ng / m L) There was significant difference in m NSS score (4.5 ± 0.71) vs (6.0 ± 0.82), NOX2 and Nrf2 (0.93 ng / m L vs 0.87 ng / m L) between t BHQ group and SFN group. CONCLUSION: Both t BHQ and SFN can effectively reduce the body’s own oxidative stress and improve neurological function in rat TBI model, but the effect of t BHQ is better than that of SFN.