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目的探讨氨磷汀对苯诱发小鼠血液毒性的干预作用。方法将90只小鼠随机分为4组：玉米油对照组、氨磷汀对照组、染苯组和氨磷汀干预组；在给苯前及给苯25次2d后,采集标本检测各组小鼠的一般情况、外周血细胞计数和网织红细胞绝对值、骨髓有核细胞计数、骨髓象以及骨髓、肝和脾的病理形态学观察。结果染苯组小鼠与对照组相比,表现为一般情况恶化,外周血细胞减少,骨髓有核细胞数降低,差异有统计学意义(P＜0．05或P＜0．01)骨髓象和病理学观察均显示造血细胞减少,脂肪细胞和淋巴细胞等非造血细胞增加；氨磷汀干预组较染苯组外周血白细胞计数(48．19%)、血红蛋白含量(15．10%)及网织红细胞计数(19．97%)均增加,骨髓有核细胞数增加32．56%,骨髓粒细胞、巨核细胞数量分别增加35．24%和64．86%,差异有统计学意义(P＜0．05或P＜0．01)；病理学观察显示氨磷汀干预组的苯毒性损伤作用明显轻于染苯组。结论苯对小鼠具有明显的血液毒性,氨磷汀在一定程度上减轻了苯致小鼠的血液毒性,起到了一定的干预保护作用。
Objective To investigate the effects of amifostine on benzene-induced hematotoxicity in mice. Methods 90 mice were randomly divided into 4 groups: corn oil control group, amifostine control group, benzene group and amifostine intervention group; before giving benzene and giving benzene 25 times 2 days later, The general situation of mice, peripheral blood cell count and the absolute value of reticulocytes, bone marrow nucleated cell count, bone marrow as well as bone marrow, liver and spleen pathomorphological observation. Results Compared with the control group, the results showed that the number of bone marrow cells and the number of bone marrow nucleated cells decreased significantly in the group treated with benzene, the difference was statistically significant (P <0.05 or P <0.01) Pathological observations showed that hematopoietic cells decreased and non-hematopoietic cells such as adipocytes and lymphocytes increased. Amniotic fluid levels of peripheral blood leucocyte (48.19%), hemoglobin content (15.10%) and net Reticulocyte count (19.97%) were increased, the number of bone marrow nucleated cells increased 32.56%, the number of bone marrow granulocytes, megakaryocytes increased by 35.24% and 64.86%, respectively, the difference was statistically significant (P < 0.05 or P <0.01). Pathological observation showed that the amoxicillin-induced injury in amifostine-treated group was lighter than that in benzene-treated group. Conclusion Benzene has obvious hematotoxicity in mice. Amifostine can reduce the hematotoxicity induced by benzene to a certain extent and play a certain role in the intervention and protection.