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秦皮甲素和秦皮乙素是木樨科白腊树树皮所含主要有效成分,具有抗炎抗菌抑制肿瘤和调节免疫的生理活性。文献中对秦皮甲素、乙素的测定和临床应用多有报道,但对秦皮甲素、乙素在动物脏器的分布未见报道。本文在研究秦皮甲素、乙素的药代动力学之后,采用高灵敏的β-环糊精(β-CD)单分子胶束荧光法对经预处理后的内脏样品进行测定,按文献B-CD具有中空圆锥形结构,在空腔外部及空腔两端开口处属于亲水区,在空腔内属于疏水区,这种特殊结构就形成了一个单分子胶束,当外界客体分子(Guest M)的直径与主体分子(Host M)β-CD空腔直径相当时,就可以嵌入腔内,由于腔内的疏水区微环境改变就可达到增溶作用。本文选用的秦皮甲素、秦皮乙素都是客体分子,都可以嵌入β-CD空腔内形成包含物,利用β-CD包含物的增溶增敏作用,达到测定微量和痕量药物的目的。本文设计的实
Aesculin and apigenin are the main active ingredients contained in the bark of the deciduous tree, and have anti-inflammatory, antibacterial and anti-tumor activity and regulate the immune activity. In the literature, there are many reports on the determination and clinical application of cortex apigenin and B, but there is no report on the distribution of cortex A and B in animal organs. After studying the pharmacokinetics of aesculin and B, the highly sensitive β-cyclodextrin (β-CD) single-molecule micellar fluorescence method was used to measure the visceral samples after pretreatment. -CD has a hollow conical structure. It is a hydrophilic zone outside the cavity and at both ends of the cavity. It is a hydrophobic zone in the cavity. This special structure forms a single molecular micelle, when the external guest molecules ( When the diameter of Guest M) is equal to the diameter of host-molecule β-CD cavity, it can be embedded in the cavity, and solubilization can be achieved due to the change of microenvironment in the hydrophobic region of the cavity. The aesculin and apiminin used in this study are all guest molecules and can all be embedded in the β-CD cavity to form inclusions. The solubilization and sensitization effects of the β-CD inclusions can be used to determine trace and trace amounts of drugs. . The design of this article