论文部分内容阅读
目的:探讨小鼠肝脏大部分切除后再生模型的改良制作方法。方法:将8~10周龄雄性C57BL/6小鼠腹腔麻醉后,用带针6-0 proline缝线环绕肝左外叶、肝中叶共同肝蒂,距肝蒂0.2 cm穿肝右中叶表面固定,结扎肝蒂,于结扎线远侧0.2 cm处切除小鼠肝脏左外叶、肝中叶(左、右)。观察小鼠术后成活率以及术后3 d、7 d、14 d小鼠肝脏质量。结果:成功建立小鼠肝脏大部分切除模型,该模型切除小鼠肝左外叶、肝中叶(左、右),切除肝质量占肝总质量的66.7%,术后小鼠存活率100%。术后小鼠剩肝代偿性增生,术后第3天恢复至原肝总质量的58.2%,第7天恢复至原肝总质量的78.0%,术后第14天恢复至原肝总质量的93.2%。结论:本研究建立的改进型小鼠肝脏大部分切除后再生模型,具有简便、可操作性强、成功率高等优点。
OBJECTIVE: To explore an improved method of making a model for the regeneration of most of the liver of mice after resection. Methods: Male C57BL / 6 mice (8-10 weeks old) were anesthetized by peritoneal anesthesia. The 6-0 proline suture was used to surround the left lateral lobe, middle hepatic lobe and common hepatic pedicle, , Ligature the pedicle, and remove the left outer lobe and the middle lobe of the liver (left and right) in the distal part of the ligature by 0.2 cm. The survival rate of mice after operation and the liver quality of mice at 3 d, 7 d and 14 d after operation were observed. Results: Most resection models of mouse liver were successfully established. The model excised the left outer lobe of liver and middle lobe of liver (left and right), the mass of the resected liver accounted for 66.7% of the total mass of liver, and the survival rate of the mice after operation was 100%. After the operation, the remaining liver of the mice was compensated for hyperplasia, which returned to 58.2% of the total mass of the original liver on the 3rd day after operation and returned to 78.0% of the total mass of the original liver on the 7th day after operation. The total mass of the original liver returned to the 14th day after the operation Of 93.2%. CONCLUSION: Most resected and regenerated models of improved mouse liver established in this study have the advantages of simplicity, high operability and high success rate.