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目的：测定恶性疟原虫云南株富含组氨酸蛋白 Ｉ Ｉ部分序列，了解该虫株与其它虫株 Ｈ Ｒ Ｐ Ｉ Ｉ基因序列的差异。方法：采用 Ｐ Ｃ Ｒ 方法特异性扩增 Ｈ Ｒ Ｐ Ｉ Ｉ基因片段，并将该基因片段克隆于 Ｍ １３ 载体，用双脱氧链末端终止法进行测序，应用 Ｐ Ｃ Ｇ Ｅ Ｎ Ｅ软件对不同虫株恶性疟原虫 Ｈ Ｒ Ｐ Ｉ Ｉ进行基因序列分析。结果：我国云南株恶性疟原虫与 ７ Ｇ８ 及 Ｄ１０ 虫株 Ｈ Ｒ Ｐ Ｉ Ｉ基因有不同程度的差异，３ 虫株间 Ｈ Ｒ Ｐ Ｉ Ｉ氨基酸序列同源性为７０．３％ ，核苷酸序列同源性为 ６８．６％ 。结论：云南株与７ Ｇ８ 及 Ｄ１０ 虫株 Ｈ Ｒ Ｐ Ｉ Ｉ基因存在差异。
OBJECTIVE: To determine the partial sequence of histidine-rich protein I I in Yunnan strain of Plasmodium falciparum and find out the difference of H R P I I gene sequence between the strain and other strains. Methods: The specific fragment of H R P I I gene was amplified by P C R method. The gene fragment was cloned into the M 13 vector and sequenced by the dideoxy chain termination method. The software P C G E N E Plasmodium falciparum H R P I I gene sequence analysis. Results: The results showed that there was a difference in H R P I I gene between P. falciparum and 7 G8 and D10 strains in Yunnan. The homology of the H R P I I amino acid sequences among the three strains was 70.3% Sequence homology was 68.6%. Conclusion: There are differences in H R P I I gene between Yunnan strain and 7 G8 and D10 strains.