论文部分内容阅读
目的:探讨p14ARF、p16INK4a蛋白在非小细胞肺癌(NSCLC)组织中的表达、意义及相关关系。方法:采用免疫组织化学SP方法对103例NSCLC组织中p14ARF和p16INK4a蛋白的表达进行检测。结果:103例NSCLC组织中p14ARF、p16INK4a蛋白表达阴性率分别为70.87%和43.69%,差异有显著性(P<0.01)。其中35例p14ARF、p16INK4a蛋白表达共阴性,共阴性率达33.98%(35/103),鳞癌中共阴性率明显高于其它组织类型(P<0.01)。p14ARF、p16INK4a蛋白表达阴性相互间无显著相关性(P>0.05)。临床Ⅲ+Ⅳ期病例两种蛋白表达阴性率明显高于临床Ⅰ+Ⅱ期(P<0.05)。结论:NSCLC组织p14ARF、p16INK4a蛋白表达共阴性具有明显的组织学类型特异性,两种蛋白阴性表达是各自独立的事件。
Objective: To investigate the expression, significance and relationship of p14ARF and p16INK4a in non-small cell lung cancer (NSCLC). Methods: The expression of p14ARF and p16INK4a protein in 103 NSCLC tissues was detected by immunohistochemical SP method. Results: The negative rates of p14ARF and p16INK4a in 103 NSCLC tissues were 70.87% and 43.69%, respectively, with significant difference (P <0.01). Among them, the expression of p14ARF and p16INK4a were negative in all 35 cases, the common negative rate was 33.98% (35/103). The negative rate of common in squamous cell carcinoma was significantly higher than that in other tissues (P <0.01). There was no significant correlation between p14ARF and p16INK4a protein expression (P> 0.05). The negative rates of the two proteins in clinical stage Ⅲ + Ⅳ were significantly higher than those in clinical stage Ⅰ + Ⅱ (P <0.05). Conclusion: The NSCLC tissue p14ARF, p16INK4a protein expression was negative for a significant histological type specificity, the two negative expression of protein are independent events.