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作者以采自无班氏丝虫流行的印尼雅加达东郊贝卡西和西郊唐格朗两地的致乏库蚊蛹期及来自曼谷、台北、西贡的蚊卵和幼虫,分别在实验室内饲养,给羽化后3~4天的雌蚊饲以班氏微丝蚴血症(8~32条/20立方毫米)者的血液。吸血后将未感染雌蚊的子代,如上法饲以同一供血者的血液进行累代选育。弃去吸血后4天内死亡的蚊虫,对以后死亡的蚊虫作解剖检查有无发育中的幼丝虫。至吸血后7~9天杀死存活的蚊虫,解剖观察幼丝虫的发育情况,该时蚊体内的幼丝虫为易于识别的Ⅱ期幼丝虫。凡有发育的幼丝虫存在的为敏感的蚊虫。
The authors collected from Cordyceps pupae, the eastern outskirts of Jakarta, Indonesia, and Tangerang, the eastern suburbs of Jakarta, Indonesia, and mosquito eggs and larvae from Bangkok, Taipei and Sai Kung, Feeding within 3 to 4 days after eclosion to Bancrofti microfilariae (8 to 32/20 cubic mm) of blood. Vampire after the uninfected female mosquito offspring, such as the law feeding the same donor blood for generations of breeding. Abandoned 4 days after the blood-sucking mosquito, mosquitoes later died for the development of juvenile filariasis. Seven to nine days after sucking blood, the surviving mosquitoes were killed and dissected to observe the development of the young larvae. At this time, the young larvae in the mosquito body were easily identified as stage II young worm. Where there is a development of young filariasis sensitive mosquitoes exist.