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以亚硒酸钠作为白内障的诱发剂,成功地诱发10～15天龄的幼鼠形成核性白内障。观测到形成白内障后,眼组织中硒含量比对照组高约7.86倍,其眼组织和静脉血中SOD酶活性分别下降54.98%;67.52%。体外模型体系ESR实验表明:亚硒酸钠和类似于眼组织内环境的物质GSH,H_2O_2反应可产生超氧阴离子自由基(O_2~-)。由以上动物实验和体外模型体系实验的结果以及对前人工作的总结,证实了亚硒酸钠诱发幼鼠白内障的机理属活性氧自由基损伤眼组织的自由基机理。
Using sodium selenite as a cataract-inducing agent successfully induced the formation of nuclear cataracts in 10-15-day-old pups. After the formation of cataract, the content of selenium in the eye tissue was about 7.86 times higher than that in the control group, and the activity of SOD in eye tissue and venous blood decreased 54.98% and 67.52% respectively. In vitro model system ESR experiments show that: sodium selenite and similar substances in the eye tissue environment GSH, H_2O_2 can produce superoxide anion radicals (O_2 ~ -). The above animal experiments and in vitro model system experimental results and summary of previous work confirmed that the mechanism of sodium selenite induced cataract in young rats is reactive oxygen free radicals damage the eye’s free radical mechanism.