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本文对环卵沉淀试验(COP)的操作方法加以改进和简化。建立了聚氯乙烯薄膜抗原片环卵沉淀试验(PVC-COP),经过薄膜片的选择和定型、混悬虫卵抗原方法的确定、抗原片保存条件的比较以及各批制备抗原片稳定性的观察等研究,最终确定了“PVC薄膜抗原片”成品的制备标准和操作规程。 对82例粪检阳性血吸虫病患者血清的检测,获得阳性反应80例(97.6％);对361例无疫水接触史者的检测,出现假阳性反应6例(1.7％),两项结果与沿用的常规COP结果相近.对来自不同疫区(九江、孝感、常山、上海、武汉)694例血清与常规COP比较,两法之间的阳性率(t检验)和平均环沉率(X~2检验)均无显著性差异(P>0.05)。证明PVC—COP除具有较理想的敏感性、特异性外,还有省工、省时、省物和便于邮递等优点,为基层开展应用环卵沉淀试验清查病人提供了一个新途径。
In this paper, the operation method of ring-and-egg precipitation test (COP) is improved and simplified. The polyvinyl chloride film antigen-ring-ring-ovum precipitation test (PVC-COP) was established. After selecting and setting film sheets, determining the method of egg suspension antigen, comparing the storage conditions of the antigen sheets and the stability of each batch of antigen sheets Observation and other studies, and ultimately determine the “PVC film antigen film” finished product preparation standards and operating procedures. Eighty-two cases (97.6%) were positive in the serum of 82 cases of stool-positive schistosomiasis patients. There were 6 cases (1.7%) of false-positive reactions in 361 cases without any contact with water, The results of conventional COPD were similar.Comparison of 694 serum samples from different affected areas (Jiujiang, Xiaogan, Changshan, Shanghai and Wuhan) showed that the positive rate (t-test) and mean echo rate (X ~ 2 test) were no significant difference (P> 0.05). It is proved that PVC-COP has the advantages of more sensitivity and specificity, saving labor, saving time, saving resources and facilitating postal delivery. It provides a new way for grass-roots units to apply the ESR test to inventory patients.