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目的研究人巨细胞病毒(HCMV)UL139基因在先天性巨结肠(HD)临床株中的多态性,探讨其多态性与HD之间的关系。方法对53株HD患儿痉挛段肠组织标本和6株尿标本的临床株进行UL139开放阅读框(ORF)的扩增及测序。对照组为10个无症状HCMV感染患儿的尿标本。结果28个HD临床株完成测序,进化树分析结果显示UL139基因DNA序列分为3组5个基因型,G3为主要基因型(48.1%)。与对照组比较,χ2=7.378,P=0.194。24个临床株同时完成了HCMVUL144基因测序,HCMVUL144与UL139基因经Kendall等级相关分析τ=-0.114,P=0.425。不同临床分型HD分散分布于UL139各个基因型中。结论HCMVUL139基因具有高度的多态性;UL139基因分型与HD的临床分型无关;UL139基因与UL144基因无相关性。
Objective To study the polymorphism of human cytomegalovirus (HCMV) UL139 gene in Hirschsprung ’s disease (HD) and to explore the relationship between its polymorphism and HD. Methods The clinical isolates of 53 strains of spastic intestinal tissue and 6 urinary specimens from patients with HD were enrolled in the study. The open reading frame (ORF) of UL139 was amplified and sequenced. The control group consisted of 10 urine specimens from asymptomatic HCMV infected children. Results 28 HD clinical isolates were sequenced. The results of phylogenetic tree analysis showed that the DNA sequence of UL139 gene was divided into 3 genotypes and 5 genotypes, with G3 as the major genotype (48.1%). Compared with the control group, χ2 = 7.378, P = 0.194. The 24 clinical isolates of HCMVUL144 were sequenced at the same time. The Kendall rank correlation analysis between HCMVUL144 and UL139 was τ = -0.114, P = 0.425. Different clinical classification of HD distributed in the UL139 various genotypes. Conclusion HCMVUL139 gene has a high degree of polymorphism; UL139 genotyping has nothing to do with the clinical classification of HD; UL139 gene has no correlation with UL144 gene.