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目的:了解献血者中输血传播病毒(TTV)的感染状况。方法:应用套式PCR(nested-PCR)和ELISA方法同步检测了388份献血者、168份非肝炎住院患者(对照组)的血清TTV DNA和抗TTV IgG。结果:TTV基因检出率分别为献血者15.46%(ALT正常组13.37%,ALT异常组41.38%,P<0.05),对照组24.40%(ALT正常组23.78%,ALT异常组50.00%,P<0.05);556份受检血清中TTV DNA检出率为18.17%,ALT正常组16.60%,ALT异常组42.31%,P<0.05两者存在显著性差异。抗TTV IgG检出率分别为献血者13.14%(ALT正常组11.70%,ALT异常组31.03%,两者存在显著性差异,P<0.05),对照组为27.98%(ALT正常组26.83%,ALT异常组75%,P<0.05),献血者组与对照组相比TTV DNA及抗TTV IgG均存在显著性差异(P<0.05)。结论:献血者组和对照组中存在TTV感染,TTV感染与ALT异常有关,提示TTV可能有一定的致病性。TTV感染和输血安全性有待进一步研究。抗TTV IgG可用于检测TTV感染。
Objective: To understand the infection status of blood transfusion transmitted virus (TTV) in blood donors. Methods: Serum TTV DNA and anti-TTV IgG were simultaneously detected by nested-PCR and ELISA in 388 blood donors and 168 non-hepatitis inpatients (control group). Results: The detection rates of TTV gene were 15.46% (13.37% in ALT normal group, 41.38% in ALT abnormal group, P <0.05), 24.40% in control group (23.78% in ALT normal group and 50.00% in ALT abnormal group, P < 0.05). The detection rate of TTV DNA in 556 sera was 18.17%, 16.60% in ALT normal group and 42.31% in ALT abnormal group, P <0.05. The detection rate of anti-TTV IgG were 13.14% of blood donors (ALT normal group 11.70%, ALT abnormal group 31.03%, there was a significant difference between the two, P <0.05), control group 27.98% (ALT normal group 26.83%, ALT (75%, P <0.05). There was significant difference between TTV DNA and anti-TTV IgG in blood donors group and control group (P <0.05). Conclusion: There is TTV infection in blood donor group and control group. TTV infection is associated with abnormal ALT, suggesting that TTV may have certain pathogenicity. TTV infection and blood transfusion safety needs further study. Anti-TTV IgG can be used to detect TTV infection.