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目的:初步探讨乙肝病毒基因变异、基因型及亚型与肝细胞癌(HCC)发病的相关性。方法:特异探针杂交法检测乙肝病毒C启动子变异,分别用特异探针杂交和特异引物PCR两种方法鉴定病毒基因型,限制性酶切片段长度多态性法鉴定部分HCC乙肝病毒的基因亚型。结果:HCC组与非HCC(NHCC)组HBV-DNA载量无明显差异,两组HBV中A1762T/G1764A变异株分别占77.8%和44.4%。B和C型为HBV主要基因型,基因型B和C在HCC患者中分别为3例(11.1%)和24例(88.9%),在NHCC患者中分别为29例(42.6%)和35例(51.5%),HCC组24例基因型C的HBV中21例为C2亚型。结论:乙肝病毒C启动子A1762T/G1764A双变异、基因型C与HCC的发生密切相关,HCC患者HBV基因亚型主要为C2亚型。
OBJECTIVE: To investigate the relationship between genetic variants, genotypes and subtypes of hepatitis B virus and the incidence of hepatocellular carcinoma (HCC). METHODS: Specific probe hybridization was used to detect the mutation of hepatitis C virus C promoter. The virus genotypes were identified by specific probe hybridization and specific primer PCR, respectively. Partial HCC hepatitis B virus genes were identified by restriction fragment length polymorphism. Subtypes. Results: There was no significant difference in HBV-DNA load between HCC and non-HCC (NHCC) groups. The A1762T/G1764A variants accounted for 77.8% and 44.4% of HBV respectively. Types B and C were the major genotypes of HBV, genotypes B and C were 3 (11.1%) and 24 (88.9%) in HCC patients, respectively, and 29 (42.6%) and 35 patients in NHCC respectively. (51.5%) Of the 24 HCC patients with genotype C, 21 were C2 subtypes. Conclusion: The double mutation of the hepatitis B virus C promoter A1762T/G1764A and genotype C are closely related to the occurrence of HCC. The HBV genotype of HCC patients is mainly C2 subtype.