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观察并研究了在黑龙江大兴安岭地区首次发现、分离的落叶松毛虫CPV的组织病理学和病毒超微结构。感染幼虫早期症状不甚明显,末期大多数肛门粘有串状白绿色粪便。病变只发生于中肠,中肠肿大、变粗,呈乳白色。感染后72h.中肠上皮细胞质中开始形成多角体;114h,中肠上皮细胞质中出现无数空洞;144h,中肠上皮细胞质中出现大量多角体,DsCPV多角体在SEMT呈六角形、球形和多边形,并以多边形为主,大小为0.63μm×1.6μm~15.7μm×1.02μm。病毒粒子为球形二十面体,表面有12根管状突起,粒子大小为16.0~58.1nm。
The histopathology and virus ultrastructure of CPV, the first isolate found in Daxinganling, Heilongjiang Province, were observed and studied. Infected larvae early symptoms are not obvious, the end of most anal sticky white string of stool. Lesions occur only in the midgut, midgut swollen, thicker, milky white. 72h after infection. In the midgut epithelial cytoplasm, polyhedrosis began to form in the cytoplasm; 114h, numerous vacuoles appeared in the cytoplasm of midgut epithelium; 144h, there were a large number of polyhedra in the cytoplasm of midgut epithelium; DsCPV polyhedra were hexagonal, spherical and polygonal in SEMT, The main size is 0.63μm × 1.6μm ~ 15.7μm × 1.02μm. Virus particles spherical icosahedron, the surface of 12 tubular protrusions, the particle size of 16.0 ~ 58.1nm.