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目的:了解深圳地区流感样病例的病毒病原学现况,为呼吸道传染病的诊断和预防控制提供病原学依据。方法:采用实时荧光定量PCR法,对269份咽拭子样本同时检测流感病毒、腺病毒、鼻病毒、副流感病毒、呼吸道合胞病毒、冠状病毒和人偏肺病毒共7种15个亚型常见呼吸道病毒。结果:2011年深圳流感样病例的病毒阳性检出率为50.2%(135/269),在检出的呼吸道病毒中鼻病毒检出最多,其次是流感病毒、冠状病毒和腺病毒。夏季和秋季都是鼻病毒占优势,分别为57.6%(19/33)和52.6%(20/38);冬季则以流感病毒为优势,占60.5%(23/38)。儿童中鼻病毒检出率最高,青少年和成人中流感病毒检出率最高。结论:鼻病毒、流感病毒、冠状病毒和腺病毒是深圳地区流感样病例的主要病原体,随着季节、月份的不同和年龄的不同而具有一定的流行规律。
Objective: To understand the status of virus etiology in influenza-like cases in Shenzhen and provide etiological evidence for the diagnosis and prevention and control of respiratory infectious diseases. Methods: Real-time fluorescence quantitative PCR method was used to detect 7 kinds of 15 subtypes of influenza virus, adenovirus, rhinovirus, parainfluenza virus, respiratory syncytial virus, coronavirus and human metapneumovirus simultaneously in 269 throat swab samples Common respiratory virus. Results: The positive rate of influenza virus in Shenzhen in 2011 was 50.2% (135/269). Among the detected respiratory viruses, rhinovirus was the most frequently detected, followed by influenza virus, coronavirus and adenovirus. Rhinovirus was predominant in summer and autumn, accounting for 57.6% (19/33) and 52.6% (20/38) respectively, while in winter, influenza virus predominates, accounting for 60.5% (23/38). Children have the highest detection rate of mid-rhinovirus and the highest detection rate of influenza virus among adolescents and adults. Conclusion: Rhinovirus, influenza virus, coronavirus and adenovirus are the major pathogens of influenza-like cases in Shenzhen. They have certain epidemic patterns with different seasons, months and ages.