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目的:了解EB病毒(Epstein-Barr virus,EBV)相关胃癌(EBVaGC)和阴性胃癌(EBVnGC)组织中视网膜母细胞瘤(Rb)基因甲基化状态及蛋白表达,探讨EBV感染与Rb基因甲基化的关系。方法:采用甲基化特异性PCR(MSP)对各种临床病理指标匹配的23例EBVaGC和25例EBVnGC组织及相应癌旁组织中Rb基因启动子区域的甲基化状态进行检测,并采用免疫组化技术检测两种胃癌组织中Rb蛋白的表达。结果:胃癌与相应癌旁组织中Rb基因启动子区的甲基化率分别为64.6%(31/48)和39.6%(19/48),差异有显著性(P<0.05);EBVaGC组织中Rb基因启动子区的甲基化率为82.6%(19/23),高于EBVnGC中的检出率(48.0%,12/25),差异有显著性(P<0.05);EBVaGC和EBVnGC组织中Rb蛋白的表达率分别为52.2%(12/23)和72.0%(18/25),差异无显著性(P﹥0.05);胃癌组织中Rb启动子基因甲基化与蛋白表达无明显负相关。结论:Rb异常甲基化在胃癌细胞中较常见,EBV可以诱导Rb基因甲基化,影响其基因表达而参与EBVaGC的发生。
OBJECTIVE: To investigate the methylation status and protein expression of retinoblastoma (Rb) gene in Epstein-Barr virus (EBV) -related gastric cancer (EBVaGC) and negative gastric cancer (EBVnGC) and to investigate the relationship between EBV infection and Rb gene methyl The relationship between. Methods: Methylation-specific PCR (MSP) was used to detect the methylation status of Rb gene promoter region in 23 cases of EBVaGC and 25 cases of EBVnGC matched with clinicopathological parameters, and immunohistochemistry Histochemistry was used to detect the expression of Rb protein in two gastric cancer tissues. Results: The methylation rates of Rb gene promoter region in gastric cancer tissues and corresponding paracancerous tissues were 64.6% (31/48) and 39.6% (19/48), respectively, with significant difference (P <0.05). In EBVaGC tissues The methylation rate of Rb gene promoter region was 82.6% (19/23), which was significantly higher than that of EBVnGC (48.0%, 12/25) (P <0.05). EBVaGC and EBVnGC tissues Rb protein expression in gastric cancer tissues was 52.2% (12/23) and 72.0% (18/25), respectively, with no significant difference (P> 0.05). The methylation and protein expression of Rb promoter in gastric cancer tissues were not significantly negative Related. Conclusion: Abnormal methylation of Rb is more common in gastric cancer cells. EBV can induce methylation of Rb gene and affect its gene expression, which is involved in the development of EBVaGC.