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目的 :研究肠三叶因子 (ITF)在水浸束缚应激 (WRS)大鼠胃粘膜基因表达变化 ,探讨其在应激胃粘膜损伤早期修复作用。 方法 :采用水浸束缚应激制作模型 ,动态监测胃粘膜血流量 (GMBF) ,大体及光镜下观察粘膜损伤程度 (UI)及组织学变化 ;逆转录 多聚酶链反应 (RT PCR)检测ITF基因表达变化 ;免疫组化染色进一步证实其表达。 结果 :应激造成胃粘膜广泛损伤 ,但损伤指数在 2、4、8h逐渐减小 ,至 8h降为 6 4 .9% ,GMBF逐渐恢复 ,至 8h上升为正常 (89.8% ) ,ITF基因表达逐渐增强 (0 .0 2 2± 0 .0 0 1 )vs(0 .1 77± 0 .0 1 0 ) ,P <0 .0 1 ,免疫组化染色计分为 (0 .1 34± 0 .0 0 1 )vs(0 .2 5 3± 0 .0 1 ) ,P <0 .0 1。 结论 :ITF可能参与了胃粘膜早期重建
Objective: To investigate the gene expression changes of intestinal trefoil factor (ITF) in gastric mucosa of rats under water immersion restraint stress (WRS), and to investigate its role in the early repair of gastric mucosal injury induced by stress. Methods: The gastric mucosal blood flow (GMBF) was dynamically monitored by immersion restraint stress model. The degree of mucosal injury (UI) and histological changes were observed under light microscope and general microscope. The ITF gene was detected by reverse transcriptase polymerase chain reaction (RT PCR) Expression changes; immunohistochemical staining further confirmed its expression. Results: The gastric mucosal injury was extensively induced by stress, but the injury index decreased gradually at 2, 4 and 8 hours and decreased to 64.9% at 8 hours. The GMBF gradually recovered and rose to normal level at 8 hours (89.8%). ITF gene expression (0. 12 ± 0. 001) vs (0. 77 ± 0. 0 1 0), P <0. 01, and the score of immunohistochemical staining was (0 .1 34 ± 0 .0 0 1) vs (0 .2 5 3 ± 0 .0 1), P <0 .0 1. Conclusion: ITF may be involved in the early reconstruction of gastric mucosa