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目的:研究NaCl对酸浆试管苗生长及生理特性的影响,以探讨其耐盐性。方法:在附加0.2%~1.0%NaCl的胁迫培养基上对试管苗进行培养,测定其脯氨酸、可溶性糖和可溶性蛋白、叶绿素、丙二醛(MDA)含量和质膜相对透性及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)的活性,并对其进行分析。结果:当NaCl为0.6%及以下时,随浓度的增加酸浆试管苗株高、茎粗、根长、生根数及叶绿素含量均逐渐降低,而当NaCl为0.8%及以上时,试管苗株高、茎粗和叶绿素含量急剧下降,根的发生被显著抑制;脯氨酸含量及SOD活性均随NaCl浓度的增加呈上升趋势,可溶性糖和可溶性蛋白及CAT和POD活性均随NaCl浓度的升高呈先上升后下降的趋势,但可溶性糖和可溶性蛋白含量均高于对照;MDA含量和质膜透性在0.6%NaCl及以下时积累缓慢,而NaCl为0.8%及以上时急剧增加。结论:酸浆试管苗具有适应一定浓度(≤0.6%NaCl)的盐渍生境能力,在其受到盐胁迫时可通过提高体内渗透调节物质含量和抗氧化酶活性来缓解盐害。
Objective: To study the effects of NaCl on the growth and physiological characteristics of test-tube plantlets in order to investigate its salt-tolerance. Methods: Plantlets were cultured in stress medium supplemented with 0.2% -1.0% NaCl. The content of proline, soluble sugar, soluble protein, chlorophyll, malondialdehyde (MDA) Oxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity, and its analysis. Results: When NaCl was 0.6% and below, plant height, stem diameter, root length, root number and chlorophyll content decreased gradually with the increase of concentration, but when NaCl was 0.8% and above, The contents of soluble sugar, soluble protein and CAT and POD were all increased with the increase of NaCl concentration The content of soluble sugar and soluble protein were higher than that of the control; MDA content and plasma membrane permeability accumulated slowly at 0.6% NaCl and below, but sharply increased when NaCl was 0.8% and above. CONCLUSION: Physalis testis plantlets have the ability of adapting to saline habitat at a certain concentration (≤0.6% NaCl) and can alleviate the salt damage by increasing osmotic adjustment substance content and antioxidant enzyme activity in vivo.