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目的探讨基质细胞衍生因子-1α(SDF-1α)及白细胞介素-1β(IL-1β)诱导内皮细胞表达淋巴管表型的作用。方法 SDF-1α和IL-1β分别诱导内皮细胞株CRL-1730,用Real-time PCR、Western blotting及免疫细胞化学等方法检测其内皮及淋巴管标志物,的表达情况。结果 SDF-1α诱导培养之后,CRL-1730细胞株的内皮细胞标志物血管性血友病因子(vWF)、血管内皮钙黏蛋白(VE-cadherin)、血管内皮生长因子受体(VEGFR)2随其浓度增高而表达降低,淋巴管标志物平足蛋白(podoplanin)、同源异形盒蛋白-1(Prox-1)和淋巴管内皮透明质酸受体-1(LYVE-1)随其浓度增高而表达增高。IL-1β诱导之后,CRL-1730细胞株的vWF、VEGFR2和podoplanin、prox-1、LYVE-1的变化趋势同SDF-1α,而VE-cadherin的表达量基本不变。结论 SDF-1α和IL-1β都能够诱导血管内皮细胞表达淋巴管标志物。
Objective To investigate the role of SDF-1α and IL-1β in the expression of lymphatic vessels in endothelial cells. Methods Endothelial cell line CRL-1730 was induced by SDF-1α and IL-1β respectively. The expression of endothelial and lymphatic markers was detected by Real-time PCR, Western blotting and immunocytochemistry. Results After induction of SDF-1α, the endothelial cell markers of vWF, VE-cadherin and VEGFR2 in CRL-1730 cells were The concentration of lymphatic vessel markers podoplanin, Prox-1 and LYVE-1 increased with the increase of their concentrations The expression increased. The changes of vWF, VEGFR2 and podoplanin, prox-1 and LYVE-1 in CRL-1730 cell line were similar to that of SDF-1α induced by IL-1β, but the expression of VE-cadherin remained unchanged. Conclusion Both SDF-1α and IL-1β can induce the expression of lymphatic vessel markers in vascular endothelial cells.