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目的:探讨血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)1型受体(angiotensinⅡtype 1 receptor,AT1R)在人胃癌细胞株中的表达及AngⅡ对AT1R高表达胃癌细胞(MKN-28)增殖和周期的影响。方法 :采用Western印迹法检测AT1R在胃癌细胞中的表达。应用不同浓度的AngⅡ(10-10~10-5mol/L)、AT1R阻滞剂(氯沙坦)以及AngⅡ2型受体(angiotensinⅡtype 2 receptor,AT2R)阻滞剂(PD123319)作用于MKN-28细胞,以CCK-8法测定各处理组细胞的相对数目,计算增殖促进效应;应用流式细胞仪检测各处理组细胞周期变化,计算各处理因素对细胞周期的影响。结果:AT1R在多数胃癌细胞株中表达,其中MKN-28细胞株表达量最高,AngⅡ可明显促进MKN-28细胞增殖以及G1期细胞向S、G2期转化,氯沙坦可明显抑制AngⅡ对MKN-28细胞的促增殖及促进G1期细胞向S、G2期转化的作用,PD123319无此作用。结论:AngⅡ通过AT1R明显促进MKN-28细胞增殖,促进G1期细胞向S、G2期转化。
Objective: To investigate the expression of angiotensin Ⅱ type 1 receptor (AT1R) in human gastric cancer cell lines and the effect of Ang Ⅱ on the proliferation and cell cycle of AT1R-overexpressing gastric cancer cells (MKN-28). Methods: Western blotting was used to detect the expression of AT1R in gastric cancer cells. MKN-28 cells were treated with various concentrations of AngⅡ (10-10 ~ 10-5mol / L), AT1R blockers (losartan) and angiotensin Ⅱ type 2 receptor (AT2R) The relative number of cells in each treatment group was determined by CCK-8 method to calculate the proliferation promoting effect. The changes of cell cycle in each treatment group were detected by flow cytometry, and the influence of each treatment factor on cell cycle was calculated. Results: AT1R was expressed in most gastric cancer cell lines, of which MKN-28 cells had the highest expression level, AngⅡcan significantly promote the proliferation of MKN-28 cells and G1, S phase and G2 phase cells. Losartan significantly inhibited the effect of AngⅡon MKN -28 cells and promote the G1 phase cells to S, G2 phase transformation, PD123319 no such effect. CONCLUSION: AngⅡ can significantly promote the proliferation of MKN-28 cells through AT1R and promote the G1 phase cells to transform into S and G2 phases.