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目的观察转染微小RNA(miR)-126 mimics/inhibitor对人类急性早幼粒细胞白血病细胞系(HL-60)增殖与凋亡的影响。方法培养HL-60细胞,RT-PCR测定miR-126相对表达水平,利用瞬时质粒转染技术,转染miR-126 mimics/inhibitor,通过CCK-8,流式细胞技术及克隆形成实验检测HL-60细胞增殖、凋亡能力。结果 miR-126 mimics组0 h、24 h、48 h、72 h细胞增殖能力显著减低(P<0.05);G_1期、S期、G_2期细胞增殖明显受抑制(P<0.05);晚期凋亡及早期凋亡(UR+LR)凋亡率增高(P<0.05);平均克隆形成率显著减低(P<0.05);miR-126 inhibitor组0 h、24 h、48 h、72 h细胞增殖能力明显增强(P<0.05);G_1期、S期、G_2期增殖能力增强(P<0.05);UR+LR明显减低(P<0.05);平均克隆形成率显著升高(P<0.05)。结论 miR-126能够抑制HL-60细胞增殖能力,促进细胞凋亡,可能作为抑癌性的微RNA在白血病的发生发展中发挥作用。
Objective To investigate the effects of microRNA (miR) -126 mimics / inhibitor on the proliferation and apoptosis of human acute promyelocytic leukemia cell line (HL-60). Methods The HL-60 cells were cultured and the relative expression level of miR-126 was determined by RT-PCR. MiR-126 mimics / inhibitor was transfected by miR-126 mimics / inhibitor by transient plasmid transfection and the cell viability was detected by CCK- 60 cell proliferation, apoptosis. Results The proliferation of miR-126 mimics group was significantly reduced at 0 h, 24 h, 48 h, 72 h (P <0.05). The proliferation of G_1 phase, S phase and G_2 phase was significantly inhibited (P <0.05) (P <0.05). The average colony formation rate was significantly decreased (P <0.05). The proliferation of miR-126 inhibitor group at 0 h, 24 h, 48 h, 72 h (P <0.05). The proliferation of G 1, S and G 2 phases was enhanced (P <0.05). UR + LR was significantly decreased (P <0.05). The average clone formation rate was significantly increased (P <0.05). Conclusion miR-126 can inhibit the proliferation of HL-60 cells and promote the apoptosis of cells. It may play a role as a tumor suppressor microRNA in the development of leukemia.