论文部分内容阅读
目的：探讨子宫内膜异位症（ＥＭ）患者的腹腔液（ＰＦ）对自然杀伤（ＮＫ）细胞的作用。方法：以Ｈｅｌａ细胞作为靶细胞，采用ＬＤＨ释放法测定与ＥＭ 患者ＰＦ共同孵育后正常人血ＮＫ细胞活性的变化。结果：在作用２ ｈ 时，非ＥＭ 患者和ＥＭ 患者ＰＦ对正常人外周血ＮＫ 细胞活性的抑制率分别为（３７．１±１８．９）％ 和（６５．２±２１．８）％ ，两者相差非常显著（Ｐ＜ ０．０１）。抑制率与ＥＭ 临床分期呈显著正相关（ｒ＝ ０．６０２ ３， Ｐ＜ ０．０５）。延长ＰＦ与淋巴细胞的作用时间，则ＰＦ对ＮＫ 细胞活性的抑制率也随之升高。结论：ＥＭ 患者的ＰＦ对ＮＫ 细胞活性具有明显的抑制作用。
Objective: To investigate the effect of peritoneal fluid (PF) on natural killer (NK) cells in patients with endometriosis (EM). Methods: Hela cells were used as target cells, and the changes of NK cell activity in normal human blood were measured by LDH release assay. Results: The inhibitory rates of PF on NK cell activity in peripheral blood of non-EM patients and EM patients were (37.1 ± 18.9)% and (65.2 ± 21.8)% at 2 h, respectively. The difference between the two groups was significant (P <0.01). The inhibition rate was positively correlated with the clinical stage of EM (r = 0.602 3, P <0.05). Prolonging the action time of PF and lymphocytes, then the inhibitory rate of PF on NK cell activity will also increase. CONCLUSION: PF in EM patients has a significant inhibitory effect on NK cell activity.