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研究组蛋白去乙酰化酶抑制剂SAHA联合溶瘤腺病毒ZD55-IL-24对结肠癌SW480细胞的体外杀伤作用。采用MTT法、结晶紫实验检测SAHA、ZD55-IL-24以及二者联合使用对结肠癌细胞株SW480及人正常肺上皮细胞株Beas-2B的增殖抑制作用;利用Hoechst33342染色对经各种处理的细胞进行凋亡形态学观察,采用流式细胞术对凋亡进行量化;通过Western blot法在蛋白水平上检测SW480细胞中IL-24的表达情况。结果显示SAHA与ZD55-IL-24联合处理对SW480的增殖抑制作用明显优于两者单独使用。10 MOI病毒ZD55-IL-24与0.5μmol/L SAHA联合作用4天,SW480细胞存活率仅为12%,明显低于10 MOI病毒单独处理组细胞的存活率(40%,P<0.05)。然而,正常细胞对于联合给药显示出良好的耐受性。Hoechst33342染色和流式细胞术结果也表明联合处理组的SW480细胞凋亡特征更明显。此外,IL-24在ZD55-IL-24病毒单独感染组及病毒与药物联合组的SW480细胞中均能有效表达。
To investigate the in vitro cytotoxicity of histone deacetylase inhibitor SAHA combined with oncolytic adenovirus ZD55-IL-24 on human colon cancer SW480 cells. The inhibitory effect of SAHA, ZD55-IL-24 and their combination on the proliferation of colon cancer cell line SW480 and human normal lung epithelial cell line Beas-2B were detected by MTT assay and crystal violet assay. The Hoechst33342 staining The morphological changes of the cells were observed and the apoptosis was quantified by flow cytometry. The expression of IL-24 in SW480 cells was detected by Western blot. The results showed that SAHA and ZD55-IL-24 combined treatment of SW480 significantly inhibited the proliferation of the two alone. 10 MOI virus ZD55-IL-24 combined with 0.5μmol / L SAHA for 4 days, the survival rate of SW480 cells was only 12%, which was significantly lower than that of 10 MOI virus alone treatment groups (40%, P <0.05). However, normal cells show good tolerability for combination administration. The results of Hoechst33342 staining and flow cytometry also showed that the apoptotic characteristics of SW480 cells in the combined treatment group were more obvious. In addition, IL-24 was efficiently expressed in SW480 cells infected with ZD55-IL-24 alone or in combination with virus and drug.