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克隆肿瘤相关抗原基因，从分子水平上研究其结构与功能，对于深入了解肿瘤的发生机制很有帮助。本研究利用自制的肝癌特异性单克隆抗体（ｍＡｂ） Ｆ１１，筛选肝癌细胞ｃＤＮＡ表达文库，获得１个阳性克隆，并对其进行了序列测定及同源性比较分析。１材料和方法１． １材料抗
The cloning of tumor-associated antigen genes and the study of their structure and function at the molecular level are helpful for further understanding the mechanism of tumorigenesis. In this study, self-made liver cancer-specific monoclonal antibody (mAb) F11 was used to screen the cDNA expression library of hepatocellular carcinoma cells. One positive clone was obtained, and sequence analysis and homology comparison analysis were performed. 1 Materials and Methods 1. 1 Material resistance