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目的调查分析龙岩市2015年布鲁氏菌病暴发疫情,为防控提供科学依据。方法采用现场流行病学调查方法,对病例发病情况及感染来源进行分析,应用血培养、血清试管凝集试验和RT-PCR对血液标本进行检测。结果共发现5例布鲁氏菌病例,血清抗体滴度均≥1∶800;对5例可疑病例进行布鲁氏菌分离培养,分离到2株羊种布鲁氏菌;对4例可疑病例血浆样本及血液增菌培养物进行RT-PCR检测,1例布鲁氏菌阳性。结论 2015年龙岩市5例布鲁氏菌病疫情,因养殖户接触病羊导致感染。
Objective To investigate and analyze the outbreak of Brucellosis in Longyan City in 2015 and provide a scientific basis for prevention and control. Methods The field epidemiological investigation method was used to analyze the incidence of cases and the source of infection. Blood culture, serum test tube agglutination test and RT-PCR were used to detect the blood samples. Results A total of 5 cases of brucellosis were found, and the serum antibody titers were all ≥1: 800. Brucellosis was isolated from 5 cases of suspicious cases, and 2 strains of brucella were isolated. Four suspicious cases Plasma samples and blood enrichment cultures were detected by RT-PCR, 1 case of brucella positive. Conclusion In 2015, 5 cases of Brucellosis in Longyan City were infected because of contact with diseased sheep.