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目的:探讨ADAM23,αvβ3在大肠癌中的表达及其与肝转移等临床病理因素的关系。方法:应用免疫组化和RT-PCR方法检测53例大肠癌及其癌旁组织及20例良性病变组织中ADAM23,αvβ3的表达情况,并分析两者的蛋白表达与临床病理因素的关系。结果:大肠癌组织中ADAM23蛋白与mRNA阳性表达率明显低于癌旁组织与良性病变组织(均P<0.05),而αvβ3蛋白与mRNA阳性表达率明显高于癌旁组织与良性病变组织(均P<0.05)。大肠癌组织中ADAM23和αvβ3蛋白的表达呈负相关(χ2=10.3390;r=-0.637,P<0.01)。ADAM23和αvβ3的蛋白表达与患者的性别,年龄及分化程度无关(均P>0.05),而与临床分期,淋巴转移及肝转移有关,此外ADAM23蛋白的表达还与大肠癌浸润深度有关(均P<0.05)。结论:大肠癌中ADAM23呈低表达,αvβ3呈高表达,两者可能与大肠癌的肝转移等不良临床转归有关。
Objective: To investigate the expression of ADAM23 and αvβ3 in colorectal carcinoma and its relationship with clinicopathological factors such as liver metastasis. Methods: The expressions of ADAM23 and αvβ3 in 53 cases of colorectal carcinoma and its adjacent tissues and 20 cases of benign lesions were detected by immunohistochemistry and RT-PCR. The relationship between the expression of ADAM23 and αvβ3 was analyzed. Results: The positive rates of ADAM23 protein and mRNA in colorectal cancer tissues were significantly lower than those in paracancerous tissues and benign lesions (all P <0.05), while the positive rates of αvβ3 protein and mRNA were significantly higher than those in paracancerous tissues and benign lesions P <0.05). The expression of ADAM23 and αvβ3 in colorectal cancer tissues was negatively correlated (χ2 = 10.3390; r = -0.637, P <0.01). The protein expression of ADAM23 and αvβ3 was not related to the gender, age and differentiation of patients (all P> 0.05), but was related to clinical stage, lymph node metastasis and liver metastasis. In addition, the expression of ADAM23 protein was also related to the depth of invasion <0.05). Conclusion: The expression of ADAM23 in colorectal cancer is low, and αvβ3 is highly expressed, both of which may be related to adverse clinical outcomes such as liver metastasis of colorectal cancer.