Molecular Characterization for Leaf Curl Virus Resistance in Cotton

• 作 者: J.AMUDHA G.BALASUBRAMANI C.D.MAYEE
• 机 构: Central Institute for Cotton Research ICAR,Nagpur-440010,India,Central Institute for Cotton Research
• 出 处: 棉花学报
• 发表日期: 2002年S1期
• 关键词: similarity Cotton cotton primer susceptible DNA sequenced designated have

RAPD analysis was carried out in germplasmlines CNH 123(RCLCuV),CNH 1012(RCLCuV),CNH 1020(SCLCuV)and CNH120(SCLCuV)to establish polymorphismamong the cotton leaf curl virus(CLCuV)resistant and susceptible genotypes.These lineswere characterized using 80 decamer primers byamplification.Eighty primers amplified 392scorable DNA fragments out of which 20% werepolymorphic.The lines CNH 123 and CNH 120have similarity(85%)as obtained by the Neiand Lies coefficient of similarity.The primer

RAPD analysis was carried out in germplasm lines CNH 123 (RCLCuV), CNH 1012 (RCLCuV), CNH 1020 (SCLCuV) and CNH120 (SCLCuV) to establish polymorphismamong the cotton leaf curl virus (CLCuV) resistant and susceptible genotypes.These lineswere characterized using 80 decamer primers by amplification.Eighty primers amplified 392 scorable DNA fragments out of which 20% werepolymorphic.The lines CNH 123 and CNH 120 similarity (85%) as obtained by the Neiand Lies coefficient of similarity.The similarity. primer