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将含无启动子报告基因的基因诱捕载体转染ES细胞，体外筛选内源基因的插入突变．建成嵌合小鼠，可以方便地研究突变基因的时空表达特征及时胚胎早期发育的影响；根据突变基因与报告基因形成的融合转录子，又为其克隆和进一步分析提供了重要依据．基于区细胞和转基因小鼠途径基因诱捕技术的发展为深入认识哺乳动物发育的分子机制和基因调控提供了一种独特的工具，并已取得了重要成果．
The ES cells were transfected with the gene-trapping vector containing the promoterless reporter gene and the insertion mutation of the endogenous gene was screened in vitro. The establishment of a chimeric mouse can conveniently study the temporal and spatial expression characteristics of the mutant gene and the influence of early embryonic development. The fusion transcripts formed by the mutant gene and the reporter gene provide an important basis for their cloning and further analysis. The development of gene-trapping techniques based on the pathway-specific cellular and transgenic mouse pathways provides a unique tool for understanding the molecular mechanisms and gene regulation of mammalian development in depth and has yielded important results.