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果糖-1,6-二磷酸醛缩酶(FBA)是糖酵解、糖异生、磷酸戊糖途径及光合作用过程的关键酶。本研究利用荧光定量分析研究 IbFBA5基因在甘薯不同组织及不同生长时期的表达及与叶片和贮藏根中碳水化合物积累的关系。结果表明, IbFBA5具有组织表达特异性,在甘薯‘徐781’叶片中表达量最高,其次是地下部(牛蒡根、储藏根和纤维根),叶柄、茎和花中表达量较低;甘薯储藏根初始膨大期至膨大盛期, IbFBA5在‘徐薯18’叶片中表达量均极显著高于在‘徐781’中的表达量;而在储藏根初始膨大期和膨大盛期,该基因在‘徐781’储藏根中的表达量均显著或极显著高于‘徐薯18’中的表达量。可溶性糖和淀粉含量分析表明,甘薯初始膨大期至膨大中期,叶片和储藏根中果糖和葡萄糖的含量均极显著提高,储藏根中淀粉含量也极显著提高,然而储藏根中的蔗糖含量及叶片中淀粉含量极显著降低。甘薯叶片和储藏根中 IbFBA5的表达模式分别与不同时期叶片中淀粉的含量变化和储藏根中果糖及葡萄糖的含量变化趋势具有一致性。
Fructose-1,6-bisphosphate aldolase (FBA) is a key enzyme in glycolysis, gluconeogenesis, pentose phosphate pathway and photosynthesis. In this study, we used fluorescence quantitative analysis to study the expression of IbFBA5 gene in different tissues and growth stages of sweet potato and the relationship with carbohydrate accumulation in leaves and storage roots. The results showed that IbFBA5 had the highest expression level in tissues, the highest expression was in leaves of ’Xu 781’, followed by the underground parts (burdock root, storage root and fiber root), and lower in petiole, stem and flower. The expression of IbFBA5 in ’Xuushu 18’ leaves was significantly higher than that in ’Xu 781’ during the initial swollen-up to swollen-blooming periods, whereas in the early swollen- The expression level of ’Xu 781’ in storage roots was significantly or extremely significantly higher than that in ’Xushu 18’. The content of soluble sugar and starch showed that the contents of fructose and glucose in leaves and storage roots were significantly increased and the starch content in storage roots was also significantly increased during the initial swelling period to the mid-expansion stage of sweet potato. However, the contents of sucrose in leaves and leaves The starch content was significantly reduced. The expression patterns of IbFBA5 in leaves and storage roots of sweet potato were consistent with the change of starch content in leaves and the content of fructose and glucose in stored roots respectively.