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以二倍体马铃薯抗青枯病基因型ED13、CE171和感青枯病基因型ED25的茎段为外植体,在优化植物生长调节剂配比的基础上,对不同基因型的组培再生进行了研究。结果表明:不同基因型具有不同生长调节剂配比要求。固体—液体培养基双层看护培养对于二倍体马铃薯茎段愈伤组织的诱导有效,以固体培养基中NAA2.0mg·L-1∶6-BA2.0mg·L-1的配比为佳。对于ED13而言,液体培养基中以2,4-D2.5mg·L-1∶KT0.5mg·L-1的配比为佳,相比之下CE171则以2,4-D1.5mg·L-1∶KT1.0mg·L-1为最好,二者的愈伤诱导率均达100%;对于基因型ED25,只有在NAA2.0mg·L-1∶6-BA1.0mg·L-1和2,4-D1.5mg·L-1∶KT1.0mg·L-1时才能形成较好的愈伤。ZT在愈伤组织诱导芽分化过程中具有重要作用,单独使用ZT浓度为1.0mg·L-1时,ED13和CE171的芽分化率均达95%以上;而ED25则只有在ZT和6-BA配合使用,配比为ZT0.5mg·L-1∶6-BA2.5mg·L-1时才可获得较高的芽分化率。
The stems of diploid potato bacterial wilt resistance genotypes ED13, CE171 and susceptible bacterial wilt genotype ED25 were used as explants. Based on the optimized ratio of plant growth regulators, different genotypes of tissue culture regeneration Were studied. The results showed that: different genotypes have different growth regulator ratio requirements. The solid-liquid medium double-layer nursing training is effective for the induction of diploid potato stem callus, and the ratio of NAA 2.0 mg · L-1: 6-BA 2.0 mg · L -1 in solid medium is better . For ED13, the 2,4-D2.5mg · L-1: KT0.5mg · L-1 ratio in liquid medium is better than that of CE131 compared with 2,4-D1.5mg · L-1: KT1.0mg · L-1 was the best, both callus induction rate reached 100%; for genotype ED25 only NAA2.0mg · L-1: 6-BA1.0mg · L- 1 and 2,4-D1.5mg · L-1: KT1.0mg · L-1 can form better callus. ZT played an important role in callus induction of bud differentiation. When ZT concentration was 1.0 mg · L-1 alone, the bud differentiation rates of ED13 and CE171 reached more than 95% With the use of the ratio of ZT0.5mg · L-1: 6-BA2.5mg · L-1 when you get higher bud differentiation rate.