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目的探讨抑癌基因RASSF2A表达水平及其基因异常甲基化与喉癌的关系及意义。方法采用RT-PCR及甲基化特异性PCR(MSP)检测50例喉癌组织(实验组)和10例癌旁正常黏膜组织(对照组)中RASSF2A基因mRNA的表达水平及其启动子甲基化状态。结果喉癌组织中有54%(27/50)存在RASSF2A基因启动子,而对照组正常黏膜组织中未发现RASSF2A基因甲基化现象,差异有统计学意义(χ~2=9.818,P=0.002)。RASSF2A甲基化水平程度与喉癌患者临床病理特征无明显相关性。喉癌组织中RASSF2A基因mRNA的表达水平明显低于正常黏膜组织(χ~2=9.818,P=0.002)。RASSF2A基因甲基化的喉癌组织中其mRNA的表达减少,反之亦然。结论喉癌组织中RASSF2A基因Cp G岛甲基化与其基因表达缺失有关,可能参与了喉癌的发生发展过程。
Objective To investigate the relationship between the expression of RASSF2A gene and its abnormal methylation and laryngeal cancer and its significance. Methods RT-PCR and methylation-specific PCR (MSP) were used to detect the expression of RASSF2A mRNA in 50 cases of laryngeal carcinoma (experimental group) and 10 cases of adjacent normal mucosa (control group) State of affairs Results RASSF2A gene promoter was found in 54% (27/50) of the laryngeal cancer tissues, while methylation of RASSF2A gene was not found in the normal mucosa of the control group (χ ~ 2 = 9.818, P = 0.002 ). There was no significant correlation between the degree of methylation of RASSF2A and the clinicopathological features of laryngeal cancer patients. The expression of RASSF2A mRNA in laryngeal carcinoma was significantly lower than that in normal mucosa (χ ~ 2 = 9.818, P = 0.002). RASSF2A gene methylation in laryngeal cancer tissue mRNA expression decreased, and vice versa. Conclusion The methylation of CpG island of RASSF2A gene in laryngeal carcinoma is related to the lack of gene expression, which may be involved in the development and progression of laryngeal carcinoma.