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将肿瘤细胞直接固定在硝酸纤维素膜上,经蛋白酶消化后直接与地高辛配基(digoxigenin)标记的癌基因探针进行DNA-RNA杂交,可检测该细胞中癌基因表达的情况。本法不需提取RNA,操作简便,灵敏度高,重复性好,不需复杂的设备,更由于地高辛配基标记的探针克服了同位素标记探针半衰期短、对人体有害等缺点,有希望得到推广使用。 将肿瘤细胞PLA801-D95(人肺巨细胞癌细胞株,由中国人民解放军总医院提供)制成细胞悬液,按
Tumor cells were directly fixed on a nitrocellulose membrane and directly digested with digoxigenin and labeled with oncogene probes for DNA-RNA hybridization to detect the expression of oncogenes in the cells. This method does not need to extract RNA, easy to operate, high sensitivity, good repeatability, without complex equipment, but also because of digoxigenin-labeled probe to overcome the short half-life of the isotope labeled probe, harmful to humans and other shortcomings, there Hope to be promoted. The tumor cells PLA801-D95 (human lung giant cell carcinoma cell lines, provided by the Chinese People’s Liberation Army General Hospital) made of cell suspension, press